Hired by Carnegie's Department of Terrestrial Magnetism in 1965, Rubin developed an interest in the movement of galaxies and realised, along with her colleagues, that the speed of stars did not obey the known laws of gravity. This led her to conclude that 90 percent of the universe is made up of invisible dark matter. The theory of dark matter was first proposed by the Swiss astrophysicist Fritz Zwicky en 1933, but Rubin's work confirmed it.
As a younger student, Rubin was shut out of Princeton University's prestigious astronomy program because it only accepted men, but she went on to receive degrees at the universities of Cornell and Georgetown. An impassioned feminist, Rubin in 1965 became the first woman allowed access to the Palomar Observatory in California. In 1993 she received the National Medal of Science - America's highest scientific honour - from then president Bill Clinton.